4FJ3
14-3-3 isoform zeta in complex with a diphoyphorylated C-RAF peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-02-14 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.9778 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 72.140, 102.430, 113.890 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.730 - 1.950 |
R-factor | 0.1611 |
Rwork | 0.159 |
R-free | 0.20800 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.026 |
RMSD bond angle | 1.724 |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0093) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 20.000 | 2.000 |
High resolution limit [Å] | 1.950 | 10.000 | 1.950 |
Rmerge | 0.052 | 0.034 | 0.436 |
Number of reflections | 61869 | 523 | 4451 |
<I/σ(I)> | 19.57 | 38.04 | 4.31 |
Completeness [%] | 99.5 | 96.7 | 99.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 0.1 M Na-acetate pH 7.0, 0.8 M NaH2PO4 and 1.2 M K2HPO4 , VAPOR DIFFUSION, SITTING DROP, temperature 277K |