4FIY
Crystal Structure of GlfT2 Complexed with UDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-12-18 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.922 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 150.398, 150.398, 147.378 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 3.100 |
| R-factor | 0.211 |
| Rwork | 0.207 |
| R-free | 0.28711 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4fix |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.129 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.210 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.125 | 0.010 |
| Number of reflections | 31773 | |
| <I/σ(I)> | 22 | 1.9 |
| Completeness [%] | 99.8 | 99.2 |
| Redundancy | 8.8 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 7% PEG 4000, 100 mM sodium acetate, 5 mM TCEP, 10% (w/v) glycerol, 0.2% Triton X-100, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
