4FIX
Crystal Structure of GlfT2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-18 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 1.0442 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 150.791, 150.791, 148.016 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.450 |
| R-factor | 0.18657 |
| Rwork | 0.185 |
| R-free | 0.22228 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.054 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.540 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.104 | 0.942 |
| Number of reflections | 59863 | |
| <I/σ(I)> | 27.2 | 2 |
| Completeness [%] | 99.9 | 99.4 |
| Redundancy | 11.9 | 9.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 7% (w/v) PEG 4000, 100 mM sodium cacodylate pH 6.5, 100 mM sodium acetate, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
