4FIU
The structure of hemagglutinin of H16 subtype influenza virus with V327G mutation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.070, 240.783, 69.479 |
| Unit cell angles | 90.00, 119.68, 90.00 |
Refinement procedure
| Resolution | 42.498 - 1.999 |
| R-factor | 0.1949 |
| Rwork | 0.193 |
| R-free | 0.22820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.182 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 1.999 | 2.000 |
| Number of reflections | 132280 | |
| Completeness [%] | 99.9 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.1M Imidazole, 20% (v/v) Jeffamine ED-2001, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
