4FI9
Structure of human SUN-KASH complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-03-08 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97915 |
Spacegroup name | H 3 2 |
Unit cell lengths | 79.835, 79.835, 280.221 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 3.050 |
R-factor | 0.24381 |
Rwork | 0.241 |
R-free | 0.31031 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.338 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.100 |
High resolution limit [Å] | 3.050 | 3.050 |
Rmerge | 0.120 | 0.248 |
Number of reflections | 6763 | |
<I/σ(I)> | 21.5 | 3.5 |
Completeness [%] | 97.9 | 100 |
Redundancy | 6.4 | 6.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 297 | 50 mM MgCl2, 100 mM HEPES, 6% PEG5000 mme, 19.5 mM HECAMEG, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 297K |