4FHY
Crystal structures of the Cid1 poly (U) polymerase reveal the mechanism for UTP selectivity - Mg 3'-dATP bound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-10-30 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.9718 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 53.680, 62.520, 111.210 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.560 - 2.700 |
R-factor | 0.21487 |
Rwork | 0.212 |
R-free | 0.26931 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4fh3 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.222 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | REFMAC |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.600 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Number of reflections | 10732 | |
<I/σ(I)> | 11.28 | 2.28 |
Completeness [%] | 99.6 | 99.7 |
Redundancy | 5 | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9.5 | 298 | 100 mM CHES, pH 9.5, 5-10% w/v PEG3350, VAPOR DIFFUSION, SITTING DROP, temperature 298K |