4FHW
Crystal structures of the Cid1 poly (U) polymerase reveal the mechanism for UTP selectivity - MgGTP bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-03-21 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9786 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.413, 62.897, 111.436 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.170 - 2.500 |
| R-factor | 0.19905 |
| Rwork | 0.196 |
| R-free | 0.24893 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4fh3 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.169 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | REFMAC |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.200 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 13540 | |
| <I/σ(I)> | 13.8 | 4.43 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 6.4 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9.5 | 298 | 100 mM CHES, pH 9.5, 5-10% w/v PEG3350, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
