4FHG
Spore photoproduct lyase C140S mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-03-20 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.9786 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 54.210, 63.090, 143.290 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.350 - 2.000 |
R-factor | 0.211 |
Rwork | 0.209 |
R-free | 0.24523 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | wt structure without substrate |
RMSD bond length | 0.006 |
RMSD bond angle | 0.987 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.350 | 2.100 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.078 | 0.045 |
Number of reflections | 33427 | |
Completeness [%] | 98.2 | 96.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 200 mM Li-sulfate, 100 mM Tris/HCl, 19-27% PEG800, 70 mM HEGA-8, anaerobically , pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |