4FH1
S. cerevisiae Ubc13-N79A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-03-11 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 77.026, 77.026, 68.350 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.350 - 2.610 |
| R-factor | 0.2199 |
| Rwork | 0.219 |
| R-free | 0.24553 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jbb |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.045 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.710 | 2.700 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Number of reflections | 7065 | |
| <I/σ(I)> | 12.3 | |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 10.3 | 296 | 0.1 M CHES, pH 10.3, 20% PEG8000, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
