4FGW
Structure of Glycerol-3-Phosphate Dehydrogenase, GPD1, from Sacharomyces Cerevisiae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 70 |
Detector technology | PIXEL |
Detector | PSI PILATUS 6M |
Spacegroup name | P 43 |
Unit cell lengths | 64.420, 64.420, 198.410 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.400 - 2.450 |
R-factor | 0.2 |
Rwork | 0.200 |
R-free | 0.20750 |
Structure solution method | Phaser |
Starting model (for MR) | 1x0v |
RMSD bond length | 0.008 |
RMSD bond angle | 1.190 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.400 | 2.510 |
High resolution limit [Å] | 2.450 | 2.450 |
Rmerge | 0.510 | |
Number of reflections | 29348 | |
<I/σ(I)> | 14.24 | 3.05 |
Completeness [%] | 99.7 | 99.9 |
Redundancy | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 12% Polyethylene Glycol 8000, 0.1 M Tris-HCl pH 8.5 and 0.3 M MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |