4FGQ

Legionella pneumophila LapG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-12-02
Detector	ADSC QUANTUM 210
Wavelength(s)	0.9771
Spacegroup name	P 21 21 2
Unit cell lengths	100.929, 105.110, 43.311
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.335 - 1.645
R-factor	0.19
Rwork	0.189
R-free	0.22020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	model built based on lower resolution SAD dataset
RMSD bond length	0.006
RMSD bond angle	1.095
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.710
High resolution limit [Å]	1.645	1.650
Number of reflections	55010
Completeness [%]	96.8	88.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	278	0.14 M Ammonium Tartrate dibasic, 20% PEG3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 278K