4FGO
Legionella pneumophila LapG (calcium-bound)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-05-04 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9771 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 59.951, 59.951, 110.379 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.619 - 1.903 |
| R-factor | 0.2599 |
| Rwork | 0.259 |
| R-free | 0.26670 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.111 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 16128 | |
| Completeness [%] | 97.7 | 81.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 278 | 0.14 M Ammonium Tartrate dibasic, 20% PEG3350, 2 mM calcium chloride, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 278K |
