4FGI
Structure of the effector - immunity system Tse1 / Tsi1 from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-02-13 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 41 2 2 |
| Unit cell lengths | 97.560, 97.560, 423.740 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.330 - 3.200 |
| R-factor | 0.226 |
| Rwork | 0.224 |
| R-free | 0.26309 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.970 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.400 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.138 | 0.450 |
| Number of reflections | 35044 | |
| <I/σ(I)> | 17 | 6.48 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 14.4 | 14.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 100 mM sodium cacodylate pH 6.5, 250 mM ammonium sulfate, 18% (w/v) PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
