4FGI
Structure of the effector - immunity system Tse1 / Tsi1 from Pseudomonas aeruginosa
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-02-13 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.9794 |
Spacegroup name | P 41 2 2 |
Unit cell lengths | 97.560, 97.560, 423.740 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.330 - 3.200 |
R-factor | 0.226 |
Rwork | 0.224 |
R-free | 0.26309 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.006 |
RMSD bond angle | 0.970 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | SHELXD |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.400 |
High resolution limit [Å] | 3.200 | 3.200 |
Rmerge | 0.138 | 0.450 |
Number of reflections | 35044 | |
<I/σ(I)> | 17 | 6.48 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 14.4 | 14.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 100 mM sodium cacodylate pH 6.5, 250 mM ammonium sulfate, 18% (w/v) PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 293K |