4FGD
Structure of the effector protein Tse1 from Pseudomonas aeruginosa, selenomethionine variant
Experimental procedure
| Experimental method | SAD |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-02-13 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.210, 108.400, 83.760 |
| Unit cell angles | 90.00, 93.80, 90.00 |
Refinement procedure
| Resolution | 45.450 - 2.600 |
| R-factor | 0.2079 |
| Rwork | 0.206 |
| R-free | 0.25112 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.103 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.700 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.092 | 0.333 |
| Number of reflections | 21546 | |
| <I/σ(I)> | 8.4 | |
| Completeness [%] | 99.5 | 95.9 |
| Redundancy | 9.3 | 8.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 200 mM KSCN, 1% (v/v) MPD, and 15% (w/v) PEG 3350, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
