4FFY
Crystal structure of DENV1-E111 single chain variable fragment bound to DENV-1 DIII, strain 16007.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-08-19 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 135.330, 135.330, 52.250 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.350 - 2.500 |
| R-factor | 0.176 |
| Rwork | 0.174 |
| R-free | 0.22900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.883 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.092 | 0.494 |
| Number of reflections | 17132 | |
| <I/σ(I)> | 18.78 | 2.35 |
| Completeness [%] | 98.1 | 94.3 |
| Redundancy | 6.2 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.4 | 293 | 20% PEG 3350, 0.2M Potassium sulfate, and 5% glycerol, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
