4FFV

Crystal Structure of Dipeptidyl Peptidase IV (DPP4, DPP-IV, CD26) in Complex with 11A19 Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2010-01-15
Detector	ADSC QUANTUM 315
Spacegroup name	P 1 21 1
Unit cell lengths	97.260, 201.720, 97.280
Unit cell angles	90.00, 93.71, 90.00

Refinement procedure

Resolution	30.000 - 2.400
R-factor	0.27241
Rwork	0.271
R-free	0.30122
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.992
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.15)
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	100.860	44.752	2.530
High resolution limit [Å]	2.400	7.590	2.400
Rmerge		0.039	0.544
Number of reflections	140149
<I/σ(I)>	9.4	14.5	1.3
Completeness [%]	96.4	89.1	96.7
Redundancy	3.3	3.2	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.9	289	2.2-2.7 M ammonium sulfate, 0.1 M Tris 7.5-7.9, vapor diffusion, temperature 289K