4FFF

Crystal Structure of Levan Fructotransferase from Arthrobacter ureafaciens

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 6C1
Synchrotron site	PAL/PLS
Beamline	6C1
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 210
Wavelength(s)	0.97949,0.97961,0.97181
Spacegroup name	P 21 21 21
Unit cell lengths	81.958, 166.625, 261.964
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.570
Rwork	0.212
R-free	0.25560
Structure solution method	MAD
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.570
Number of reflections	104570

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	295	0.1M Bis-Tris pH5.5, 1% PEG3350, 1M Ammonium sulfate, 3% 2-propanol, VAPOR DIFFUSION, HANGING DROP, temperature 295K