4FFC
Crystal structure of a 4-aminobutyrate aminotransferase (GabT) from Mycobacterium abscessus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-05-09 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 67.400, 67.530, 102.460 |
| Unit cell angles | 78.06, 81.26, 77.44 |
Refinement procedure
| Resolution | 50.000 - 1.800 |
| R-factor | 0.1396 |
| Rwork | 0.138 |
| R-free | 0.16690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3oks |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.392 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.3.0) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.850 | |
| High resolution limit [Å] | 1.800 | 8.050 | 1.800 |
| Rmerge | 0.038 | 0.022 | 0.123 |
| Number of reflections | 152966 | 1760 | 10080 |
| <I/σ(I)> | 29.14 | 70.77 | 8.03 |
| Completeness [%] | 96.2 | 98.9 | 85.9 |
| Redundancy | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | MyabA.01026.b.A1 PS00899 at 20 mg/mL against PACT F6 0.2 M sodium formate, 0.1 M Bis Tris Propane pH 6.5, 20% PEG 3350, crystal tracking ID 233334f6, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
