4FEZ
Inosine 5'-monophosphate dehydrogenase from Vibrio cholerae, deletion mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-04-24 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 110.005, 110.005, 166.817 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.100 - 2.160 |
| R-factor | 0.1673 |
| Rwork | 0.166 |
| R-free | 0.19280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.797 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 39.100 | 50.000 | 2.200 |
| High resolution limit [Å] | 2.160 | 5.860 | 2.160 |
| Rmerge | 0.126 | 0.033 | 0.748 |
| Number of reflections | 55638 | ||
| <I/σ(I)> | 6 | 2.04 | |
| Completeness [%] | 100.0 | 99.7 | 99.7 |
| Redundancy | 12.8 | 13 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 15% tacsimate, 0.1 M Hepes buffer, 2% PEG-3350, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
