4FE9
Crystal Structure of SusF from Bacteroides thetaiotaomicron
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 130 |
| Detector technology | CCD |
| Collection date | 2010-12-15 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97941 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 188.694, 49.148, 83.618 |
| Unit cell angles | 90.00, 97.36, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| R-factor | 0.201 |
| Rwork | 0.199 |
| R-free | 0.24700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | SEMET SUSF |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.018 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 46531 | |
| <I/σ(I)> | 16.9 | 3.5 |
| Completeness [%] | 89.1 | 77.3 |
| Redundancy | 2.5 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.3 | 292 | 6-12% glycerol, 1.5-2M Na/KPO4, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
