4FDY

Crystal structure of a similar to lipoprotein, NLP/P60 family (SAV0400) from Staphylococcus aureus subsp. aureus Mu50 at 2.23 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-04-05
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97895,0.91837,0.97853
Spacegroup name	P 1 21 1
Unit cell lengths	40.666, 71.994, 124.917
Unit cell angles	90.00, 95.91, 90.00

Refinement procedure

Resolution	47.035 - 2.230
R-factor	0.2006
Rwork	0.199
R-free	0.24180
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.040
Data reduction software	XDS
Data scaling software	XSCALE (December 29, 2011)
Phasing software	SHELX
Refinement software	BUSTER-TNT (2.10.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.035		2.310
High resolution limit [Å]	2.230	4.780	2.230
Rmerge	0.052	0.030	0.719
Number of reflections	33428	3381	3371
<I/σ(I)>	12.6	31	1.7
Completeness [%]	94.9	92	96.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	277	0.2M calcium acetate, 20.0% polyethylene glycol 8000, 0.1M MES pH 6.0, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K