4FDK

F78L Tt H-NOX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-12-11
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.000
Spacegroup name	P 21 21 2
Unit cell lengths	80.552, 127.426, 42.935
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.971 - 2.100
R-factor	0.1728
Rwork	0.170
R-free	0.23090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1U55 CHAIN A
RMSD bond length	0.014
RMSD bond angle	1.392
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.140
High resolution limit [Å]	2.100	2.100
Number of reflections	26525
<I/σ(I)>		3
Completeness [%]	100.0	100
Redundancy		4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.4		0.2 M sodium citrate, pH 4.4, 25% w/v PEG3350, 25 mg/mL protein in 20 mM TEA, pH 7.5, VAPOR DIFFUSION, SITTING DROP