4FDF
Structural insights into putative molybdenum cofactor biosynthesis protein C (MoaC2) from Mycobacterium tuberculosis H37Rv
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-18 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 91.900, 91.900, 91.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.710 - 2.202 |
| R-factor | 0.2075 |
| Rwork | 0.205 |
| R-free | 0.24942 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ekr |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.116 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0111) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.710 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 13345 | |
| Completeness [%] | 99.9 | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | 0.025M potassium sodium tartrate tetra hydrate solution, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
