4FDB

Three-dimensional structure of the protein prib from ralstonia solanacearum at the resolution 1.8a. northeast structural genomics consortium target rsr213c

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4C
Synchrotron site	NSLS
Beamline	X4C
Temperature [K]	100
Detector technology	CCD
Collection date	2012-04-11
Detector	MAR CCD 165 mm
Wavelength(s)	0.979
Spacegroup name	I 41 2 2
Unit cell lengths	70.661, 70.661, 141.117
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.776 - 1.800
R-factor	0.1968
Rwork	0.196
R-free	0.21750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3en2
RMSD bond length	0.007
RMSD bond angle	1.167
Refinement software	PHENIX (dev_1269)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.800
Rmerge	0.095
Number of reflections	31660
<I/σ(I)>	9.3
Completeness [%]	100.0
Redundancy	26.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.5	277	Protein solution: 100mM NaCl, 5mM DTT, 0.02% NaN3, 10mM Tris-HCl, pH 7.5. Reservoir solution: 5.76M K-ACETATE 0.1M NA3-CITRATE, UNDER OIL BATCH, temperature 277K