4FCX
S.pombe Mre11 apoenzym
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-01-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 76.408, 82.262, 164.296 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 21.842 - 3.000 |
| R-factor | 0.2324 |
| Rwork | 0.227 |
| R-free | 0.28580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.210 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.000 | 3.180 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 21028 | |
| Completeness [%] | 98.0 | 97.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 20mM HEPES 300mM NaCl, 0.1mM EDTA, 1mM MnCl2, 2mM beta-mercaptoethanol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
