4FCS
The crystal structures of several mutants of pleurotus eryngii versatile peroxidase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-04-20 |
Detector | ADSC QUANTUM 4r |
Wavelength(s) | 0.933 |
Spacegroup name | I 41 |
Unit cell lengths | 96.271, 96.271, 98.907 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.140 - 1.500 |
R-factor | 0.15142 |
Rwork | 0.150 |
R-free | 0.17618 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2vka |
RMSD bond length | 0.036 |
RMSD bond angle | 2.856 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.140 | 1.580 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.062 | 0.454 |
Number of reflections | 71863 | |
<I/σ(I)> | 17.2 | 3.3 |
Completeness [%] | 99.9 | 99.5 |
Redundancy | 5 | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 295 | 1.4 M ammonium sulfate, 0.1 M sodium cacodilate and 2% 1,3-propanedio, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K |