4FCH
Crystal Structure SusE from Bacteroides thetaiotaomicron with maltoheptaose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2011-06-29 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97856 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.735, 63.914, 156.828 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.300 |
| R-factor | 0.16506 |
| Rwork | 0.164 |
| R-free | 0.17737 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | SeMet SusE |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.031 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.320 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Number of reflections | 141909 | |
| <I/σ(I)> | 39.5 | 4 |
| Completeness [%] | 97.4 | 91.3 |
| Redundancy | 6.9 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 292 | 100mM Bis Tris Propane, 2.0M ammonium sulfate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
