4FCG
Structure of the leucine-rich repeat domain of the type III effector XCV3220 (XopL)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-20 |
| Detector | SBC-3 |
| Wavelength(s) | 0.97937 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 50.463, 95.228, 115.465 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.932 - 2.000 |
| R-factor | 0.1736 |
| Rwork | 0.171 |
| R-free | 0.22580 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.071 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXCD |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.072 | 0.364 |
| Number of reflections | 19207 | |
| <I/σ(I)> | 38.4 | 4.9 |
| Completeness [%] | 99.9 | 99.3 |
| Redundancy | 7.3 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 295 | 0.2M Potassium sulfate, 20% PEG3350, cryoprotected with 10% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 295K, pH 6.7 |
