4FCC
Glutamate dehydrogenase from E. coli
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-03-17 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97918 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 105.218, 176.262, 150.250 |
Unit cell angles | 90.00, 89.94, 90.00 |
Refinement procedure
Resolution | 49.653 - 2.000 |
R-factor | 0.1818 |
Rwork | 0.181 |
R-free | 0.22900 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.028 |
Data reduction software | DENZO |
Data scaling software | SCALA (3.3.20) |
Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 49.653 | 49.653 | 2.070 |
High resolution limit [Å] | 1.999 | 7.740 | 2.000 |
Rmerge | 0.125 | 0.590 | |
Total number of observations | 23876 | 107166 | |
Number of reflections | 365923 | ||
<I/σ(I)> | 5.1 | 4.7 | 1.2 |
Completeness [%] | 99.2 | 98.9 | 92.6 |
Redundancy | 3.7 | 3.7 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 0.1 M Bis-Tris/HCl pH 6.5, 0.2 NH4Ac, 19% PEG6000, VAPOR DIFFUSION, HANGING DROP, temperature 291.0K |