4FCA
The crystal structure of a functionally unknown conserved protein from Bacillus anthracis str. Ames.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-04-28 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 72.279, 156.180, 48.225 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.673 - 2.055 |
| R-factor | 0.2099 |
| Rwork | 0.208 |
| R-free | 0.25000 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.050 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.000 | 2.090 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.097 | 0.574 |
| Number of reflections | 34563 | |
| <I/σ(I)> | 29.7 | 2.8 |
| Completeness [%] | 99.3 | 99.5 |
| Redundancy | 5.4 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 0.16M Magnesium Chloride, 0.08M Tris:HCl, 24% (w/v) PEG4000, 20% (v/v) Glycerol, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
