4FC9
Structure of the C-terminal domain of the type III effector Xcv3220 (XopL)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-13 |
| Detector | SBC-3 |
| Wavelength(s) | 0.97921 |
| Spacegroup name | P 32 |
| Unit cell lengths | 119.198, 119.198, 38.679 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.630 - 1.800 |
| R-factor | 0.154 |
| Rwork | 0.152 |
| R-free | 0.19570 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.759 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXCD |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.831 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.063 | 0.519 |
| Number of reflections | 110283 | |
| <I/σ(I)> | 25 | 1.85 |
| Completeness [%] | 99.4 | 99 |
| Redundancy | 3.2 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | 0.2 M Sodium Acetate, 30% PEG 4K, 4% ethylene glycol, 0.1 M Tris 8.5, cryoprotected with Paratone-N oil, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
