4FC8
Crystal structure of transcription regulator protein Rtr1 from Kluyveromyces lactis
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.2833, 1.2836, 1.2652 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.168, 88.734, 94.803 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.500 |
| R-factor | 0.225 |
| Rwork | 0.222 |
| R-free | 0.27200 |
| Structure solution method | MAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.647 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 24945 | |
| Completeness [%] | 99.1 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9.5 | 293 | 0.1M CHES pH 9.5 20% (w/v) PEG 8000, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
