4FC8

Crystal structure of transcription regulator protein Rtr1 from Kluyveromyces lactis

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2011-07-20
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.2833, 1.2836, 1.2652
Spacegroup name	P 21 21 21
Unit cell lengths	45.168, 88.734, 94.803
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.500
R-factor	0.225
Rwork	0.222
R-free	0.27200
Structure solution method	MAD
RMSD bond length	0.017
RMSD bond angle	1.647
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	REFMAC (5.5.0102)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.590
High resolution limit [Å]	2.500	2.500
Number of reflections	24945
Completeness [%]	99.1	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		9.5	293	0.1M CHES pH 9.5 20% (w/v) PEG 8000, VAPOR DIFFUSION, SITTING DROP, temperature 293K