4FC5
Crystal Structure of Ton_0340
Experimental procedure
| Experimental method | SAD |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 4A |
| Synchrotron site | PAL/PLS |
| Beamline | 4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-23 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.2822 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 107.181, 107.181, 356.384 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.450 - 2.300 |
| R-factor | 0.223 |
| Rwork | 0.223 |
| R-free | 0.28100 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.500 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 87763 | |
| Completeness [%] | 94.0 | 99.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 295 | 5% (v/v) isopropanol, 100mM sodium acetate (pH 5.0), 350mM zinc acetate, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
