4FBZ

Crystal structure of deltarhodopsin from Haloterrigena thermotolerans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-11-17
Detector	ADSC QUANTUM 315
Wavelength(s)	1
Spacegroup name	H 3 2
Unit cell lengths	111.710, 111.710, 198.250
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	15.000 - 2.700
R-factor	0.235
Rwork	0.235
R-free	0.26100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1iw6
RMSD bond length	0.007
RMSD bond angle	1.172
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	86.900
High resolution limit [Å]	2.700
Rmerge	0.075
Number of reflections	13186
<I/σ(I)>	19.4
Completeness [%]	98.7
Redundancy	7.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.6	283	membrane fusion method, pH 7.6, VAPOR DIFFUSION, SITTING DROP, temperature 283K