4FBY
fs X-ray diffraction of Photosystem II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | FREE ELECTRON LASER |
| Source details | SLAC LCLS BEAMLINE CXI |
| Synchrotron site | SLAC LCLS |
| Beamline | CXI |
| Temperature [K] | 298 |
| Detector technology | PIXEL |
| Collection date | 2011-09-19 |
| Detector | CS-PAD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 130.783, 227.764, 308.630 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 85.890 - 6.560 |
| R-factor | 0.367 |
| Rwork | 0.366 |
| R-free | 0.38500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3BZ1 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.483 |
| Data reduction software | cctbx.xfel |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 85.890 | 6.730 |
| High resolution limit [Å] | 6.556 | 6.500 |
| Number of reflections | 17962 | |
| <I/σ(I)> | 6.74 | |
| Completeness [%] | 98.1 | 89.6 |
| Redundancy | 2.84 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 298 | 4% PEG 2000, 5 mM CaCl2, 100 mM PIPES, pH 7.0, batch, temperature 298K |
