4FBV
Crystal structure of the Myxococcus Xanthus hemagglutinin in complex with a3,a6-mannopentaose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E SUPERBRIGHT |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2011-07-26 |
Detector | RIGAKU SATURN 944 |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 45.592, 57.319, 105.969 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.820 - 1.760 |
R-factor | 0.18764 |
Rwork | 0.186 |
R-free | 0.21368 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4fbr |
RMSD bond length | 0.012 |
RMSD bond angle | 1.400 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0044) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.820 | 1.820 |
High resolution limit [Å] | 1.760 | 1.760 |
Rmerge | 0.053 | 0.206 |
Number of reflections | 27394 | |
<I/σ(I)> | 22.1 | 3.8 |
Completeness [%] | 96.8 | 81.5 |
Redundancy | 6.63 | 2.53 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 0.05 M monobasic potassium phosphate and 20% w/v polyethylene glycol 3350, pH 8, VAPOR DIFFUSION, SITTING DROP, temperature 298K |