4FBV
Crystal structure of the Myxococcus Xanthus hemagglutinin in complex with a3,a6-mannopentaose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2011-07-26 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.592, 57.319, 105.969 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.820 - 1.760 |
| R-factor | 0.18764 |
| Rwork | 0.186 |
| R-free | 0.21368 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4fbr |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.400 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0044) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.820 | 1.820 |
| High resolution limit [Å] | 1.760 | 1.760 |
| Rmerge | 0.053 | 0.206 |
| Number of reflections | 27394 | |
| <I/σ(I)> | 22.1 | 3.8 |
| Completeness [%] | 96.8 | 81.5 |
| Redundancy | 6.63 | 2.53 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 0.05 M monobasic potassium phosphate and 20% w/v polyethylene glycol 3350, pH 8, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
