4FBS
Structure of monomeric NT from Euprosthenops australis Major Ampullate Spidroin 1 (MaSp1)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-07-16 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0718 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 42.650, 33.244, 44.752 |
Unit cell angles | 90.00, 115.37, 90.00 |
Refinement procedure
Resolution | 40.435 - 1.700 |
R-factor | 0.1448 |
Rwork | 0.142 |
R-free | 0.19570 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Initial NMR model of NT A72R |
RMSD bond length | 0.010 |
RMSD bond angle | 1.228 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | PHENIX ((phenix.refine: dev_1041)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.440 | 1.761 |
High resolution limit [Å] | 1.700 | 1.700 |
Number of reflections | 12385 | |
<I/σ(I)> | 5.2 | |
Completeness [%] | 97.6 | 80.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1M Bis Tris propane pH 6.5, 0.25M NaBr, 20% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K |