4FBQ

Crystal structure of a covalently fused Nbs1-Mre11 complex with two manganese ions per active site

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2009-03-29
Detector	PSI PILATUS 6M
Wavelength(s)	1.006
Spacegroup name	P 21 21 21
Unit cell lengths	59.256, 79.032, 222.968
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.410 - 2.500
R-factor	0.2154
Rwork	0.213
R-free	0.25690
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.133
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall
Low resolution limit [Å]	47.410
High resolution limit [Å]	2.492
Number of reflections	36665
Completeness [%]	97.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP			400 mM ammonium citrate pH 6.0, 20% PEG 3350, 50 mM MnCl2, VAPOR DIFFUSION, HANGING DROP