4FBQ
Crystal structure of a covalently fused Nbs1-Mre11 complex with two manganese ions per active site
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2009-03-29 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.006 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 59.256, 79.032, 222.968 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.410 - 2.500 |
R-factor | 0.2154 |
Rwork | 0.213 |
R-free | 0.25690 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.133 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 47.410 |
High resolution limit [Å] | 2.492 |
Number of reflections | 36665 |
Completeness [%] | 97.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 400 mM ammonium citrate pH 6.0, 20% PEG 3350, 50 mM MnCl2, VAPOR DIFFUSION, HANGING DROP |