4FBO
Crystal structure of the Pseudomonas fluorescens agglutinin (PFA)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-03-08 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 58.982, 70.343, 62.307 |
| Unit cell angles | 90.00, 111.73, 90.00 |
Refinement procedure
| Resolution | 28.140 - 1.700 |
| R-factor | 0.18015 |
| Rwork | 0.176 |
| R-free | 0.21523 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3s5v |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.343 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0044) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.140 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.294 | |
| Number of reflections | 24397 | |
| <I/σ(I)> | 2.4 | |
| Completeness [%] | 93.8 | 90.6 |
| Redundancy | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 1.0 M sodium citrate and 0.1 M imidazole, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
