4FBL
LipS and LipT, two metagenome-derived lipolytic enzymes increase the diversity of known lipase and esterase families
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-09 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.87 |
| Spacegroup name | P 4 |
| Unit cell lengths | 105.270, 105.270, 120.980 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.300 - 1.990 |
| R-factor | 0.17805 |
| Rwork | 0.176 |
| R-free | 0.21679 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1tqh |
| RMSD bond length | 0.029 |
| RMSD bond angle | 2.193 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.300 | 2.100 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Rmerge | 0.110 | 0.800 |
| Number of reflections | 85910 | |
| <I/σ(I)> | 9.8 | 2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.1 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.5 | 292 | 0.1 M NaAcetate, 3 M NaCl,0.01 M spermidine, pH 4.5, VAPOR DIFFUSION, temperature 292K |
