4FBH
Structure of RIP from barley seeds
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | OTHER |
Temperature [K] | 290 |
Detector technology | AREA DETECTOR |
Collection date | 1992-01-01 |
Wavelength(s) | 1.5418 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 88.360, 62.590, 53.180 |
Unit cell angles | 90.00, 108.62, 90.00 |
Refinement procedure
Resolution | 25.481 - 2.300 |
R-factor | 0.185 |
Rwork | 0.178 |
R-free | 0.24360 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4fb9 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.107 |
Data reduction software | profile (fitting procedure) |
Phasing software | PHASER |
Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 32.000 | 32.000 | 2.600 |
High resolution limit [Å] | 2.300 | 15.000 | 2.300 |
Number of reflections | 11804 | ||
Completeness [%] | 89.5 | 97.7 | 80.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.41 | 295 | 100 mM Tris-HCl, 27.5 %(w/v) PEG 1500, pH 8.41, vapor diffusion, hanging drop, temperature 295K |