4FBH
Structure of RIP from barley seeds
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | OTHER |
| Temperature [K] | 290 |
| Detector technology | AREA DETECTOR |
| Collection date | 1992-01-01 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 88.360, 62.590, 53.180 |
| Unit cell angles | 90.00, 108.62, 90.00 |
Refinement procedure
| Resolution | 25.481 - 2.300 |
| R-factor | 0.185 |
| Rwork | 0.178 |
| R-free | 0.24360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4fb9 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.107 |
| Data reduction software | profile (fitting procedure) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 32.000 | 32.000 | 2.600 |
| High resolution limit [Å] | 2.300 | 15.000 | 2.300 |
| Number of reflections | 11804 | ||
| Completeness [%] | 89.5 | 97.7 | 80.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.41 | 295 | 100 mM Tris-HCl, 27.5 %(w/v) PEG 1500, pH 8.41, vapor diffusion, hanging drop, temperature 295K |
