4FBB
Structure of mutant RIP from barley seeds in complex with adenine (AMP-incubated)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-12-16 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 131.252, 142.456, 85.064 |
| Unit cell angles | 90.00, 127.52, 90.00 |
Refinement procedure
| Resolution | 31.495 - 1.800 |
| R-factor | 0.2111 |
| Rwork | 0.210 |
| R-free | 0.24970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.065 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 4.880 | 1.800 |
| Rmerge | 0.039 | 0.023 | 0.338 |
| Number of reflections | 113785 | ||
| <I/σ(I)> | 17.9 | ||
| Completeness [%] | 99.6 | 96.9 | 99 |
| Redundancy | 4.2 | 4.1 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 295 | 0.1M phosphate-citrate, 0.2M NaCl, 20%(w/v) PEG 8000, pH 4.2, vapor diffusion, hanging drop, temperature 295K |
