4FB9

Structure of mutant RIP from barley seeds

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	CCD
Collection date	2010-06-27
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97913
Spacegroup name	C 1 2 1
Unit cell lengths	130.284, 141.908, 84.819
Unit cell angles	90.00, 126.92, 90.00

Refinement procedure

Resolution	41.295 - 1.750
R-factor	0.1711
Rwork	0.171
R-free	0.19670
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.072
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.7.3_928)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.810
High resolution limit [Å]	1.750	3.770	1.750
Rmerge	0.101	0.049	0.488
Number of reflections	123296
<I/σ(I)>	10.2
Completeness [%]	99.2	98.7	98.6
Redundancy	3.5	3.6	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	295	0.1M MES, 0.2M calcium acetate, 20%(w/v) PEG 8000, pH 6.0, vapor diffusion, hanging drop, temperature 295K