4FAQ
Structure of Oceanobacillus iheyensis group II intron in the presence of K+, Ca2+ and 5'-exon
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-11-28 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9795 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 89.835, 95.715, 225.737 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.820 - 3.110 |
R-factor | 0.20396 |
Rwork | 0.201 |
R-free | 0.25833 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3igi |
RMSD bond length | 0.007 |
RMSD bond angle | 2.483 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.190 |
High resolution limit [Å] | 3.110 | 3.110 |
Rmerge | 0.063 | 0.605 |
Number of reflections | 35518 | |
<I/σ(I)> | 12.1 | 2 |
Completeness [%] | 99.6 | |
Redundancy | 3.5 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 303 | 100 mM potassium acetate, 100 mM potassium chloride, 100 mM calcium chloride, 50 mM HEPES sodium, pH 7.0, 3% PEG8000, VAPOR DIFFUSION, HANGING DROP, temperature 303K |