4FA7
Structure of Recombinant Cytochrome ba3 Oxidase mutant A204F from Thermus thermophilus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-22 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 143.550, 98.210, 94.860 |
| Unit cell angles | 90.00, 127.92, 90.00 |
Refinement procedure
| Resolution | 74.830 - 2.500 |
| R-factor | 0.17174 |
| Rwork | 0.169 |
| R-free | 0.21969 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3s8g |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.077 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 74.830 |
| High resolution limit [Å] | 2.500 |
| Number of reflections | 33684 |
| Completeness [%] | 98.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 6.5 | 293 | 50mM Sodium Cacodylate, 1.6M NaCl, 40% PEG400, pH 6.5, Lipidic Cubic Phase, temperature 293K |
