4FA0
Crystal structure of human AdPLA to 2.65 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-01-01 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0000 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 59.991, 59.991, 74.006 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.140 - 2.650 |
R-factor | 0.2079 |
Rwork | 0.206 |
R-free | 0.24710 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2kyt |
RMSD bond length | 0.010 |
RMSD bond angle | 1.050 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER (2.3.0) |
Refinement software | BUSTER-TNT |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 51.954 | 2.390 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.052 | 0.437 |
Number of reflections | 7196 | |
<I/σ(I)> | 14.6 | 2.3 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 4.3 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 5 | 293 | 25% PEG 1500, 100mM MIB buffer, pH 5.0, vapor diffusion, temperature 293K |