4F9U
Structure of glycosylated glutaminyl cyclase from Drosophila melanogaster
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-10 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.91841 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.665, 56.964, 95.669 |
| Unit cell angles | 90.00, 108.16, 90.00 |
Refinement procedure
| Resolution | 90.900 - 1.800 |
| R-factor | 0.1706 |
| Rwork | 0.170 |
| R-free | 0.22480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3si2 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.488 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.902 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.079 | 0.514 |
| Number of reflections | 57047 | |
| <I/σ(I)> | 10.6 | 2.2 |
| Completeness [%] | 99.0 | 97.8 |
| Redundancy | 4.2 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 294 | 10% PEG35000, 2 mM PQ50, 0.1 M Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
