4F96
Crystal Structure of VldE, the pseudo-glycosyltransferase, in complex with GDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-04-02 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.821, 121.151, 84.333 |
| Unit cell angles | 90.00, 91.74, 90.00 |
Refinement procedure
| Resolution | 37.524 - 2.152 |
| R-factor | 0.1908 |
| Rwork | 0.190 |
| R-free | 0.20920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.297 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.770 | 2.230 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Number of reflections | 51924 | |
| Completeness [%] | 99.9 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 100 mM Tris-HCl, pH 8.0, 30-35% PEG3350, 3 mM GDP, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
