4F8T
Crystal Structure of the Human BTN3A3 Ectodomain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-10-09 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97857 |
Spacegroup name | P 21 2 21 |
Unit cell lengths | 39.841, 78.430, 98.412 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.964 - 2.382 |
R-factor | 0.2223 |
Rwork | 0.221 |
R-free | 0.24490 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4f80 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.163 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.7_650) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.420 |
High resolution limit [Å] | 2.380 | 6.460 | 2.380 |
Number of reflections | 12256 | ||
Completeness [%] | 94.7 | 99.3 | 66.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Sitting drop | 4 | 298 | 0.1M Citric acid pH4.0, 0.8M ammonium sulfate, 0.15M Sodium Fluoride, Sitting drop, temperature 298K |