4F8R
Bacillus DNA Polymerase I Large Fragment complex 7
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Detector technology | CCD |
Collection date | 2011-11-19 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 93.670, 109.370, 150.390 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.982 - 1.640 |
R-factor | 0.1821 |
Rwork | 0.181 |
R-free | 0.20340 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 2HVI WITH INSERTION SITE BASE PAIR METAL IONS AND PROTEIN RESIDUES 681-721 DELETED |
RMSD bond length | 0.011 |
RMSD bond angle | 1.384 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Refinement software | PHENIX (dev_1026) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.740 |
High resolution limit [Å] | 1.640 | 4.900 | 1.640 |
Rmerge | 0.038 | 0.021 | 0.476 |
Number of reflections | 172238 | 7324 | 30352 |
<I/σ(I)> | 25.19 | 63.27 | 3.98 |
Completeness [%] | 91.2 | 97.5 | 99.9 |
Redundancy | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 290 | 48%-54% Saturated Ammonium Sulfate, 2.5% MPD, 10mM MgSO4, 100mM MES, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 290K |